Bacillus subtilisから誘導したアデニンアナログ耐性株によるアデノシン生産

概要

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A mutant resistant to adenine analogs was derived from an adenosine producer, Bacillus subtilis A 3-5, which had xanthine auxotrophy, 8-azaguanine resistance, and sulfaguanidine resistance. Selection during breeding was done by screening for resistance to 2-thioadenine sulfate (0.5mg/ml), resistance to 8-azaadenine (1mg/ml), and resistance to 6-methylaminopurine (1mg/ml), in that order. The strain obtained, No.43-35, produced more adenosine than the parent strain. For increased production of adenosine, the effects of the cultural conditions were examined. Strain No.43-35 produced 24 g/l adenosine from 130 g/l glucose in a glass jar fermenter with a working volume of 300 ml at the best cultural conditions found.
社団法人日本農芸化学会の論文
1994-04-01