酵素固定化バイオリアクターによる分岐シクロデキストリンの合成

概要

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SM-10 was reported as a porous ceramic carrier that had high performance for enzyme immobilization. We constructed a bioreactor filled with SM-10 (SMBR) and used it for a synthesis reaction of branched cyclodextrin, which proceeded with a highly viscous (700 c.p.) substrate at a high temperature (60℃). We conducted a field test for branched cyclodextrin (Br-CD) synthesis using the SMBR pilot reactor. Its planning productive capacity was 1 ton/month. The results of the field test were : (1)Productive capacity of SMBR for Br-CD synthesis was 72 tons/m^3/month, about 60 times as large as one using the conventional method. (2)The stability of immobilized pullulanase was dependent not only on the operating temperature but also the substrate concentration. Highly concentrated substrate was preferable for the pullulanase stability. (3)During the field test for 700 h, we obtained the desirable Br-CD product, with a branching ratio over 52%, using the SMBR pilot reactor.
社団法人日本生物工学会の論文
1990-05-25