Aspergillus oryzaeとAspergillus kawachiiとの融合株由来の両薬剤耐性形質分離株の遺伝状況 : 麹菌のプロトプラスト融合における複核性の影響(第2報)

概要

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Cycloheximide-nystatin resistant segregants from fusants between the cycloheximide (500μg/ml) resistant strains, AOCY-5 and AOCY-6 of Aspergillus oryzae and the nystatin (420 U/ml) resistant strains AKNY-3 and AKNY-9 of Aspergillus kawachii were investigated to elucidate the genetic condition. There were three types of cycloheximide-nystatin resistant segregant according to the formation of sectors on PGA medium with or without benomyl (0.1 μg/ml).…X1 type, X2 type and X3 type. By the appearence of sectors on benomyl-plates, DNA content in conidia and the number of nuclei in conidia, X1 type seemed to be a heterokaryon, X2 type seened to be a diploid (partial) and X3 type seemed to be a balanced heterokaryon. These three types of generic condition are available to generate a cycloheximidenystatin resistant segregant owing to the multinucleate nature of Aspergillus.
公益社団法人日本生物工学会の論文
1989-03-25