Eriwinia carotovoraの2段階培養によるエンド-ペクチン酸リアーゼの生産

概要

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The productivity of endo-pectate lyase (endo-PATE)from Erwinia carotovora GIR 1044(FERM P-7576)was found to be greatly improved by two stage cultivation; in the first stage the bacterium was grown with an inducing carbon source, e.g., pectin, and in the second stage it was cultivated with glycerol, xylase, or fructose with the addition of monosodium L-glutamate as nitrogen source. In the two stage cultivation using pectin or glycerol as the carbon source the enzyme activity reached 400 units/ml, almost 3 times as much as that of one stage cultivation in a 10-l ferementor. Using two stage cultivation in the 200-l fermentator improved enzyme productivity over that in the 10-l fermentor, with 500 units/ml of activity.Compared with the cultivation in Erlenmeyer flasks, fermentor cultivation improved enzyme productivity. The optimum cultivating conditions were agitation of 480 rpm with aeration of 0.5 vvm at 28℃.
社団法人日本生物工学会の論文
1986-07-25