そ菜類軟腐病細菌の生態的研究 : 第3報 Erwinia aroideaeの検出・定量への螢光抗体法の応用

概要

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The present report describes the application of the immunofluorescent staining to the detection and the counting of Erwinia aroldeae in the presence of other bacteria. Immunofiuorescent staining was performed by indirect method. The time required for staining was 10-15 minutes. l:100 to 500 and l:32 diliutions of anti-E. aroideae 1068 serum and of labelled goat antirabbit globulin respectively were adequate for the staining of used bacterium. 2 strains of E. aroideae and 3 strains isolated from soft rotted carrots showed positive staining reactions with the foregoing antisera, while 30 strains of E. aroideae and 27 strains of bacteria belonging to 12 genera gave negative reactions under the same conditions. In impression smears from the leaves of Chinese cabbage previously sprayed with E. aroideae 1068, a number of fluorescent bacterial ce11s and colonies were recognized mainly along the joint regions of the epidermal cells. E. aroideae could be detected using the microcolony technique in the soil suspensions containing as few as 52 cells per ml. With specimens containing smaller numbers of E. aroideae, some of the promising combined methods for the calculation of this pathogen are discussed.(Received October 28,1966)
日本植物病理学会の論文
1967-06-30