〓紙電気泳動法による植物病原菌菌糸たんぱくの検索

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1) The present paper deals with the results of experiments through the paper-electrophoresis on the proteins contained in mycelia of several phytopathogenic fungi. 2) Under the current of O.25 mA/cm for 3 hours using veronal-K 2HPO 4-Na Borate buffer solution, three components of proteins (I,II,III) were recognized in mycelia of Piricularia oryzae, Cochliobolus miyabeanus, Gibberella fujikuroi, Phytophthora infestans, Phytophthora macrospora and Guignardia camelliae, but in mycelia of Corticium sasakii and Shiraia bambusicola four components, of proteins, (I,II,II',III) were recognized. 3) There were no qualitative difference between the two paper-electrograms of mycelia of different races of Piricularia oryzae, Cochliobolus miyabeanus and Gibberella fujikuroi. 4) The proteins detected by the paper-electrophoresis in mycelia of Piricularia oryzae decreased with the growing stages, and with in the extent of the writer's experiments, no electro-phoretic proteins were recognized six weeks after the inoculation. 5) Proteins, especially I and II components, contained in mycelia of Piricularia oryzae decreased if the mycelia were contacted with 10 <-4> molar solution of copper sulfate or mercuric chloride for 20 hours. The decrease of the components were probably caused by the degeneration of the proteins contacted with heavy metalic ions, but in the case of adding the metalic solutions to the juice macerated from mycelia of P. oryzae just before starting electrophoresis, the proteins did not decrease even at the concentration of 10 <-3> molar.
日本植物病理学会の論文
1961-01-25

〓紙電気泳動法による植物病原菌菌糸たんぱくの検索

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