イネ白葉枯病の病態生理に関する研究 : V. 感染イネにおける透過性の変北

概要

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Permeability changes in rice leaves inoculated with Xanthomonas campestris pv. oryzae were investigated by measuring the leakage of electrolytes and ^<14>C-assimilates to gain an understanding of the mechanisms of pathogenesis. In experiments with detached rice leaves, pieces 6 mm in length were incubated in the bacterial suspension (about 10^8 cells/ml) for 72 hr at 25 C in a shaking condition. The bacterial suspension was then replaced by distilled water, continuously shaken for an additional 4 hr at 25 C, and the conductivity of this bathing solution was measured. The electrolyte leakage was 2 to 3.5 times higher in inoculated susceptible leaf pieces than in the non-inoculated control, but was only l.4 times higher in resistant leaves. No similar increment of electrolyte leakage was observed with heat-killed bacteria, centrifuged supernatants of culture suspension or bacterial suspension to which chloramphenicol had been added. From these results, it is clearly evident that living bacteria can induce permeability enhancement of rice leaf pieces. In experiments with attached rice leaves, periodic measurement of the leakage of electrolytes and ^<14>C-assimilates revealed a significant increase in susceptible leaves from just prior to symptom development, and a slight increase from the same time in resistant leaves.
日本植物病理学会の論文
1981-09-25