イネ白葉枯病の抵抗性機作に関する研究 : IV. 感染葉の細菌抑制物質の抽出と部分的精製

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Antibacterial substances, which were produced in rice leaves after inoculation with an incompatible strain of Xanthomonas oryzae, were partially purified and some properties were examined. Main antibacterial components were extracted by ethyl acetate from acidic aqueous solution, and were active at a concentration of 250 ppm. The same fraction extracted from healthy leaves showed no activity at 250 ppm. In healthy leaves, the antibacterial activity was found in ethyl acetate extract from alkaline aqueous solution, but the activity decreased markedly after infection. Acidic antibacterial components were partially purified by Silica gel column chromatography followed by thin-layer chromatography. The main components were eluted by solvent mixture of ethyl acetate: hexane (3:7 to 1:1) from the Silica gel column. The residue of these effluents prepared from inoculated leaves showed considerable antibacterial activity at 50 ppm, while that from healthy leaves had no activity. Thin-layer chromatogram of these effluents showed that at least three antibacterial components were contained in acidic fraction, and these components seemed to be different from chlorogenic acid, caffeic acid, ferulic acid, vanillic acid, salicylic acid, p-coumaric acid or umbelliferone which were known to be present in rice leaves.
1977-11-28