トマト系タバコモザイクウイルスの弱毒系統(L_<11>A)の2・3の性質

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Some properties of an attenuated tomato strain (L_<11>A) of tobacco mosaic virus (TMV) werecompared with the parent virulent strain (L). Dilution end point of L_<11>A was 10^<-7>-10^<-8>, while L was still infective at 10^<-8>. L_<11>A lost its infectivity after heat treatment at 90 C for ten min while L did not. The infectivity of L_<11>A was lost more rapidly than that of L at 70 C. The shape of particles and the length distribution pattern of L_<11>A after purification were much the same as those of L. The sap of tomato leaves infected with L_<11>A retained about 50 % of the original infectivity after a week-storage at 25 C and less than 10 % after a year-storage, whereas that of leaves infected with L retained 80and 50 % of infectivity after respective periods. L_<11>A was suspended at 3.3mg/ml either in 0.01 M phosphate buffer (pH 7.0) or in the same buffer containing 2 % sucrose, then lyophilized and the resultant virus powder was stored at 22-25 C. The samples without sucrose completely lost their infectivities after 27 months storage, whereas the samples with sucrose were highly infectious still at the same stage of storage. To examine whether the lyophilization with sucrose affected Virulence, the sample stored for 27 months was inoculated to Nicotiana tabacum cv. Xanthi nc, and the produced lesions were isolated singly. The saps from these lesions were tested separately on tomato seedlings for their virulence. Only one lesion out of 50 produced a chlorotic patch on a leaf let of tomato. All other lesions caused no symptom. The effect of temperature on the multiplication of L_<11>A and L in tomato leaf discs was compared. The optimal temperature for L_<11>A was 25-28 C, while that for L was 28-30 C. At 35 C, L multiplied considerably but L_<11>A did not. The ratio of the amount of L_<11>A to that of L decreased consistently as the temperature was raised up to 35 C.
日本植物病理学会の論文
1979-12-26

トマト系タバコモザイクウイルスの弱毒系統(L_<11>A)の2・3の性質

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