メロンがんしゅ病放線菌の分離方法

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A method of intensive isolation for the pathogenic Streptomyces sp. causing root tumor of melon was investigated to reduce contaminative bacteria in dilution plate technique from the diseased tissues. Pre-treatment of isolating samples with phenol in 140-fold dilution for 10 minutes became extremely effective to decrease the bacterial population but efficacy of alkaline treatment with 0.01N-NaOH was low. In tested 15 inhibitors added to culture medium, 50 ppm of kanamycin had the most effect for reducing of the bacteria, and 25 ppm of kanamycin and 500 ppm of dehydroacetic acid sodium salt were secondary effective ones. The best medium for the isolation in tested 12 media was the basal medium for rhizosphere microorganisms by Lochhead and Chase (medium B), and this incubation period was 5 days at 28℃. In the isolation tests with these results from natural diseased melon, especially incorporating 50 ppm kanamycin into medium B caused the effects that not only reduced contaminative bacteria but also increased appearance of the pathogenic Streptomyces sp. in total actinomycetes. As the results, the isolation on medium B containing kanamycin (50 ppm) by incubation at 28℃ for 5 days from pretreated isolating sample with phenol dilution (×140) was best method. This constructed isolation procedure for the pathogenic Streptomyces sp. was able to enhance values of its isolation ratio (number of successful isolation tests/'number of isolation tests/100) from diseased root tumor, percentage in total actinomycetes and mean number of isolates per isolating plate in comparison with non-treated isolation on medium B : from 42.9% to 100%, from 12.5% to 71.2% and from 0.3 isolate to 80.6 isolates respectively.
日本植物病理学会の論文
1993-10-25

メロンがんしゅ病放線菌の分離方法

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