Cloning, Sequencing, and Expression of the Gene Encoding 4-Hydroxy-4-methyl-2-oxoglutarate Aldolase from Pseudomonas ochraceae NGJ1(Biochemistry & Molecular Biology)
スポンサーリンク
概要
- 論文の詳細を見る
A DNA fragment that carried the gene (proA) encoding 4-hydroxy-4-methyl-2-oxoglutarate aldolase was cloned from the chromosomal DNA of Pseudomonas ochraceae NGJ1, and the coding region was assigned to the nucleotide sequence based on the N-terminal amino acid sequence of the enzyme purified from the organism. The proA gene was 684 bp long, corresponding to a protein of 227 amino acid residues with a calculated molecular mass of 24,067 Da. The genes encoding a putative transporter and a 4-oxalomesaconate hydratase were upstream, and a 3'-truncated gene encoding 2-pyrone-4, 6-dicarboxylate lactonase was downstream from the proA gene in the same orientation on the DNA fragment. The proA gene product was overproduced in Escherichia coli and briefly purified to homogeneity from the crude extract by a two-step purification. The molecular and catalytic properties of the gene product were similar to those of the P. ochraceae enzyme.
- 社団法人日本農芸化学会の論文
- 2001-12-23
著者
-
Tsuji Nobuyuki
Department Of Biomolecular Science Faculty Of Engineering Gifu University
-
Maruyama Kiyofumi
Department Of Biomolecular Science Faculty Of Engineering Gifu University
-
Harada Toshiyuki
Department Of Biomolecular Science Faculty Of Engineering Gifu University
-
Sugisaki H
Kyoto Univ. Kyoto Jpn
-
Sugisaki Hiroyuki
Institute For Chemical Research Kyoto University
-
MIWA Michiko
Department of Biomolecular Science, Faculty of Engineering, Gifu University
-
NAGAI Tomoyuki
Department of Biomolecular Science, Faculty of Engineering, Gifu University
-
TOMITA Naotaka
Department of Biomolecular Science, Faculty of Engineering, Gifu University
-
SOBAJIMA Hirobumi
Department of Biomolecular Science, Faculty of Engineering, Gifu University
-
Miwa Michiko
Department Of Biomolecular Science Faculty Of Engineering Gifu University
-
Tomita N
T. Hasegawa Co. Ltd. Kawasaki‐shi Jpn
-
Nagai Tomoyuki
Department Of Biomolecular Science Faculty Of Engineering Gifu University
-
Sobajima Hirobumi
Biotechnology Research Center The University Of Tokyo
-
Sobajima Hirobumi
Department Of Biomolecular Science Faculty Of Engineering Gifu University
-
Tsujii Nobuyuki
Department of Biomolecular Science, Faculty of Engineering, Gifu University
関連論文
- Purification and Characterization of an Esterase Hydrolyzing Monoalkyl Phthalates from Micrococcus sp. YGJ1
- Role of Cysteine Residues in 4-Oxalomesaconate Hydratase from Pseudomonas ochraceae NGJ1
- Identification of a Jasmonic Acid-Responsive Region in the Promoter of the Rice 12-Oxophytodienoic Acid Reductase 1 Gene OsOPR1
- Cloning and Characterization of the Genes Encoding Enzymes for the Protocatechuate Meta-degradation Pathway of Pseudomonas ochraceae NGJ1
- Purification and Characterization of an Esterase from Micrococcus sp. YGJ1 Hydrolyzing Phthalate Esters(Biochemistry & Molecular Biology)
- Optimal strategy of plant antiherbivore defense : Implications for apparency and resource-availability theories
- Cloning, Sequencing, and Expression of the Gene Encoding 4-Hydroxy-4-methyl-2-oxoglutarate Aldolase from Pseudomonas ochraceae NGJ1(Biochemistry & Molecular Biology)
- The Chloride Ion Is an Environmental Factor Affecting the Biosynthesis of Pyoluteorin and 2,4-Diacetylphloroglucinol in Pseudomonas sp. YGJ3
- NONLINEAR FINITE ELEMENT ANALYSIS ON THE SHEAR STRENGTH OF RC INTERIOR BEAM-COLUMN JOINTS WITH ULTRA HIGH-STRENGTH MATERIALS
- Molecular and Catalytic Properties of 2,4′-Dihydroxyacetophenone Dioxygenase from Burkholderia sp. AZ11
- Molecular and Catalytic Properties of Monoacetylphloroglucinol Acetyltransferase from Pseudomonas sp. YGJ3
- Molecular and Catalytic Properties of 2,4'-Dihydroxyacetophenone Dioxygenase from Burkholderia sp. AZ11
- Molecular and Catalytic Properties of 2,4-Diacetylphloroglucinol Hydrolase (PhlG) from Pseudomonas sp. YGJ3
- Oxidative Degradation of 4-Hydroxyacetophenone in Arthrobacter sp. TGJ4
- Pulmonary Cryptococcosis within a Pulmonary Carcinoma-Review of Reported Cases
- Hysteroscopic Inspection and Total Curettage Are Insufficient for Discriminating Endometrial Cancer from Atypical Endometrial Hyperplasia
- Small Cell Carcinoma of the Uterine Cervix : Clinical Outcome of Concurrent Chemoradiotherapy with a Multidrug Regimen
- Scanning Removal of Ion-implanted Novolak Resist by using a Laser Irradiation