Cloning and Characterization of the Genes Encoding Enzymes for the Protocatechuate Meta-degradation Pathway of Pseudomonas ochraceae NGJ1
スポンサーリンク
概要
- 論文の詳細を見る
The 2-pyrone-4,6-dicarboxylate lactonase gene (proL), the protocatechuate 4,5-dioxygenase α and β subunits genes (proOa and proOb), and the 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase gene (proD) were cloned from the chromosomal DNA of Pseudomonas ochraceae NGJ1. These genes were in the order proLOaObD on the DNA, and a possible transcription terminator sequence followed. The proL and proD genes were over-expressed in Escherichia coli, and their gene products were purified for identification, while the expression of proOaOb was at a lower level. The protocatechuate meta-degradation operon was reconstituted with the recombinant plasmids and expressed successfully in E. coli.
- 社団法人 日本農芸化学会の論文
- 2004-07-23
著者
-
SUGISAKI Hiroyuki
Institute for Chemical Research, Kyoto University
-
Maruyama Kiyofumi
Department Of Biomolecular Science Faculty Of Engineering Gifu University
-
SHIBAYAMA Tomohiko
Department of Biomolecular Science, Faculty of Engineering, Gifu University
-
ICHIKAWA Aki
Department of Biomolecular Science, Faculty of Engineering, Gifu University
-
SAKOU Yukari
Department of Biomolecular Science, Faculty of Engineering, Gifu University
-
YAMADA Satsue
Department of Biomolecular Science, Faculty of Engineering, Gifu University
-
Sugisaki Hiroyuki
Institute For Chemical Research Kyoto University
-
Ichikawa Aki
Department Of Biomolecular Science Faculty Of Engineering Gifu University
-
Sakou Yukari
Department Of Biomolecular Science Faculty Of Engineering Gifu University
-
Yamada Satsue
Department Of Biomolecular Science Faculty Of Engineering Gifu University
-
Shibayama Tomohiko
Department Of Biomolecular Science Faculty Of Engineering Gifu University
関連論文
- Purification and Characterization of an Esterase Hydrolyzing Monoalkyl Phthalates from Micrococcus sp. YGJ1
- Role of Cysteine Residues in 4-Oxalomesaconate Hydratase from Pseudomonas ochraceae NGJ1
- Cloning and Characterization of the Genes Encoding Enzymes for the Protocatechuate Meta-degradation Pathway of Pseudomonas ochraceae NGJ1
- Purification and Characterization of an Esterase from Micrococcus sp. YGJ1 Hydrolyzing Phthalate Esters(Biochemistry & Molecular Biology)
- Cloning, Sequencing, and Expression of the Gene Encoding 4-Hydroxy-4-methyl-2-oxoglutarate Aldolase from Pseudomonas ochraceae NGJ1(Biochemistry & Molecular Biology)
- The Chloride Ion Is an Environmental Factor Affecting the Biosynthesis of Pyoluteorin and 2,4-Diacetylphloroglucinol in Pseudomonas sp. YGJ3
- Molecular and Catalytic Properties of 2,4′-Dihydroxyacetophenone Dioxygenase from Burkholderia sp. AZ11
- Molecular and Catalytic Properties of Monoacetylphloroglucinol Acetyltransferase from Pseudomonas sp. YGJ3
- Molecular and Catalytic Properties of 2,4'-Dihydroxyacetophenone Dioxygenase from Burkholderia sp. AZ11
- Molecular and Catalytic Properties of 2,4-Diacetylphloroglucinol Hydrolase (PhlG) from Pseudomonas sp. YGJ3
- Oxidative Degradation of 4-Hydroxyacetophenone in Arthrobacter sp. TGJ4