Molecular and Catalytic Properties of 2,4-Diacetylphloroglucinol Hydrolase (PhlG) from Pseudomonas sp. YGJ3
スポンサーリンク
概要
- 論文の詳細を見る
Gene phlG encoding 2,4-diacetylphloroglucinol hydrolase was cloned from Pseudomonas sp. YGJ3 and expressed in Escherichia coli. Recombinant PhlG was purified homogeneously. It required 2-mercaptoethanol for stability. Km for 2,4-diacetylphloroglucinol and kcat were determined to be 24 µM and 5.8 s−1 respectively. CoCl2 specifically and significantly activated PhlG.
- 2012-06-23
著者
-
Maruyama Kiyofumi
Department Of Biomolecular Science Faculty Of Engineering Gifu University
-
Saitou Hiroki
Department Of Biomolecular Science Faculty Of Engineering Gifu University
-
Watanabe Manami
Department Of Biomolecular Science Faculty Of Engineering Gifu University
関連論文
- Analysis of inhibition of topoisomerase IIα and cancer cell proliferation by ingenolEZ
- Purification and Characterization of an Esterase Hydrolyzing Monoalkyl Phthalates from Micrococcus sp. YGJ1
- Role of Cysteine Residues in 4-Oxalomesaconate Hydratase from Pseudomonas ochraceae NGJ1
- Cloning and Characterization of the Genes Encoding Enzymes for the Protocatechuate Meta-degradation Pathway of Pseudomonas ochraceae NGJ1
- Purification and Characterization of an Esterase from Micrococcus sp. YGJ1 Hydrolyzing Phthalate Esters(Biochemistry & Molecular Biology)
- Cloning, Sequencing, and Expression of the Gene Encoding 4-Hydroxy-4-methyl-2-oxoglutarate Aldolase from Pseudomonas ochraceae NGJ1(Biochemistry & Molecular Biology)
- The Chloride Ion Is an Environmental Factor Affecting the Biosynthesis of Pyoluteorin and 2,4-Diacetylphloroglucinol in Pseudomonas sp. YGJ3
- Molecular and Catalytic Properties of 2,4′-Dihydroxyacetophenone Dioxygenase from Burkholderia sp. AZ11
- Molecular and Catalytic Properties of Monoacetylphloroglucinol Acetyltransferase from Pseudomonas sp. YGJ3
- Molecular and Catalytic Properties of 2,4'-Dihydroxyacetophenone Dioxygenase from Burkholderia sp. AZ11
- Molecular and Catalytic Properties of 2,4-Diacetylphloroglucinol Hydrolase (PhlG) from Pseudomonas sp. YGJ3
- Oxidative Degradation of 4-Hydroxyacetophenone in Arthrobacter sp. TGJ4