Developmental Changes of Ni^<2+> Sensitivity and Automaticity in Nkx2.5-Positive Cardiac Precursor Cells From Murine Embryonic Stem Cell

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Background It is controversial which subtypes of T type Ca^<2+> channels are implicated in automaticity of cardiac cells during the embryonic period. Method and Results The effect of Ni^<2+> on the automaticity of Nkx2.5-positive cardiac precursor cells sorted from embryonic stem cells during their differentiation was examined using patch clamp techniques. Although 40μmol/L Ni^<2+>, which is enough to block Ni^<2+>sensitive T type-Ca^<2+> channels, decreased the spontaneous beating rate in all cells in the early and intermediate stage, Ni^<2+> did not show any effects on the automaticity of 50% of the cells in the late stage. Conclusion These results indicate that Ni^<2+>-sensitive T-type Ca^<2+> channels expressed in the Nkx2.5-positive cardiac precursor cells are developmentally regulated.
社団法人日本循環器学会の論文
2004-06-20