Purification and Characterization of a Metallothionein-like, Zinc-binding Protein of Scallops, Patinopecten yessoensis
スポンサーリンク
概要
- 論文の詳細を見る
A zinc-binding protein was purified to homogeneity for the first time from the gonad parts of scallops, Patinopecten yessoensis, kept in filtered seawater to which no heavy metals were added. Based upon the elution profiles in two chromatographic systems, spectrophotometric analysis, and amino acid composition of the purified preparation, the protein met the criteria for classification as a metallothionein ; i.e., low molecular weight (about 9000), paucity of both aromatic amino acid residues and absorbance at 280 nm, and abundance of both cysteinyl residues (>25%) and absorbance at 215 and 254 nm. Furthermore, the results of chromatographies on a Sephacryl S-300 column and electrophoresis with or without SDS suggested that the protein molecules would be in several polymeric forms in vivo. The antiserum prepared with the purified protein as the antigen was shown to have immunocross-reactivity to neither an extract of the surf clam, Pseudocardium sybillae, nor the whelk, Neptunea arthritica, indicating the heterogeneity of the proteins in marine shellfishes. These results suggested that the Zn-binding protein purified in this study was characteristic of scallops and involved in zinc storage of this organism.
- 社団法人日本農芸化学会の論文
- 1992-09-23
著者
-
Kikuchi Shintaro
Department of Applied Chemistry, Muroran Institute of Technology
-
OSHIMA YUKO
Department of Biological Function and Genetic Resources Science, Faculty of Agriculture, Okayama Uni
-
Oshima Y
Showa Sangyo Co. Ltd. Ibaraki Jpn
-
Oshima Yuko
Department Of Biological Function And Genetic Resources Science Faculty Of Agriculture Okayama Unive
-
Takeuchi Takao
Department of Applied Microbiology, Muroran Institute of Technology
-
Miura Toshiaki
College Of Medical Technology Hokkaido University
-
Tatewaki Masakazu
Algological Res. Inst.,Hokkaido University
-
INOHARA Mikio
Department of Applied Chemistry, Muroran Institute of Technology
-
OKAMURA Ichiei
Department of Applied Chemistry, Muroran Institute of Technology
-
Inohara Mikio
Department Of Applied Chemistry Muroran Institute Of Technology
-
Miura T
Kyushu Univ. Fukuoka Jpn
-
Okamura Ichiei
Department Of Applied Chemistry Muroran Institute Of Technology
-
Ohshima Yasuhiro
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
-
Kikuchi S
Department Of Applied Chemistry Muroran Lnstitute Of Technology
-
Kikuchi Shintaro
Department Of Applied Chemistry Muroran Institute Of Technology
-
Tatewaki Masakazu
Algological Res. Inst. Hokkaido University
-
Takeuchi Takao
Department Of Applied Chemistry Muroran Institute Of Technology
関連論文
- Molecular Cloning and Characterization of a Thermostable Carboxylesterase from an Archaeon, Sulfolobus shibatae DSM5389 : Non-Linear Kinetic Behavior of a Hormone-Sensitive Lipase Family Enzyme(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Deciphering the Molecular Basis of the Broad Substrate Specificity of α-Glucosidase from Bacillus sp. SAM1606
- Biodegradation of bisphenol A and bisphenol F in the rhizosphere sediment of Phragmites australis(ENVIRONMENTAL BIOTECHNOLOGY)
- Isolation of Iron-Oxidizing Bacteria from Corroded Concretes of Sewage Treatment Plants
- Production of α-Amylase by an Immobilized Thermophilic Bacillus Strain under Nitrate Respiration
- Motion of the Vortex Rings near the Boundary Plane of the Fluid
- Isolation of a Sulfur-oxidizing Bacterium That can Grow under Alkaline pH, from Corroded Concrete
- Structure Elucidation of Ostreocin D, a Palytoxin Analog Isolated from the Dinoflagellate Ostreopsis siamensis(Organic Chemistry)
- Numerical Simulation of Autorotation
- A New Zinc-binding Protein of Scallop, Patinopecten yessoensis, Induced under Zinc-enriched Conditions
- Purification and Characterization of a Metallothionein-like, Zinc-binding Protein of Scallops, Patinopecten yessoensis
- Diesel exhaust (DE) aggravates pathology of delayed-type hypersensitivity (DTH) Induced by methyl-bovine serum albumin (mBSA) in mice
- Structures of Azaspiracid Analogs, Azaspiracid-4 and Azaspiracid-5,Causative Toxins of Azaspiracid Poisoning in Europe
- Absolute Configuration at C45 in 45-Hydroxyyessotoxin, a Marine Polyether Toxin Isolated from Shellfish
- Extracellular Accumulation of the Polyol Lipids, 3,5-Dihydroxydecanoyl and 5-Hydroxy-2-decenoyl Esters of Arabitol and Mannitol, by Aureobasidium sp
- Thermal Convection Caused by Ring-Type Heat Source
- Simultaneous Determination of Urocanic Acid Isomers and Histidine in Human Stratum Corneum by High-Performance Liquid Chromatography
- High-performance liquid chromatographic method for rapid and highly sensitive determination of histidine using postcolumn fluorescence detection with o-phthaldialdehyde
- A novel nonsense mutation of the PEPD gene in a Japanese patient with prolidase deficiency
- Structure and Sequence of the Mouse V1a and V1b Vasopressin Receptor Genes
- Electrochemical Hydrogenation of Organic Compounds on Poly[N-(5-carboxyhexyl)pyrrole]Film-Coated Electrodes Incorporating Palladium
- Iron Storage in Mycobacterium smegmatis Grown under Iron-sufficient and Iron-overload Conditions
- Immobilization of Fatty Acid Synthetase from Mycobacterium smegmatis by Radiation-Induced Polymerization(Biological Chemistry)
- A Very Long-Chain Fatty Acid Elongation System in Mycobacterium avium and a Possible Mode of Action of Isoniazid on the System(Biological Chemistry)
- INVENTION OF SURFACE AREA CALUCULATION FORMULA FOR FEMALE ONLY
- L/N-type calcium channel blocker cilnidipine ameliorates proteinuria and inhibits the renal renin-angiotensin-aldosterone system in deoxycorticosterone acetate-salt hypertensive rats