Characterization of Carbonic Anhydrase Isozyme CA2,Which Is the CAH2 Gene Product, in Chlamydomonas reinhardtii
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概要
- 論文の詳細を見る
From high-CO_2 (5% CO_2) grown unicellular green alga, Chlamydomonas reinhardtii, carbonic anhydrase (CA) was isolated by affinity chromatography and characterized. Isolated CA was identified as an isozyme (CA2) which is the product from the second gene CAH2 by peptide sequencing. The CA2 was inactivated by dithiothreitol. This treatment caused dissociation of CA2 into the large (38 kDa) and small subunits (4243 Da). The molecular mass of the CA2 holoenzyme measured by low-angle laser light-scattering photometry and precision differential refractometry combined with gel-filtration HPLC was 87.9 kDa. These results and gene structure indicate that CA2 is a heterotetramer consisting of two large and two small subunits linked by disulfide bonds like CA1,which is the CAH1 gene product. The specific activity of CA2 purified by anion-exchange HPLC was 3300 units per mg protein, which was approximately 1.6 times higher than that of CA1. Therefore, it was concluded that two structurally related isozymes, CA1 and CA2,are present in the wild type cells of C. reinhardtii and differentially regulated by the atmospheric CO_2 concentration.
- 1992-05-23
著者
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MIYACHI SHIGETOH
Marine Biotechnology Institute
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Fukuzawa Hideya
Laboratory Of Plant Molecular Biology Department Of Agricultural Chemistry Faculty Of Agriculture Ky
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TACHIKI Akira
Life Science Research Center, Advanced Material & Technology Research Laboratories, Techinical Devel
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Miyachi Shigetoh
Marine Biotechnology Inst.
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Tachiki Akira
Life Science Research Center Advanced Material & Technology Research Laboratories Techinical Dev
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MIYACHI Shigetoh
Marine Biotechnology Institute Co., Ltd.
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FUKUZAWA Hideya
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
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