Metabolism of C_1-Compounds in Methocylated Aromatic Acid-utilizing Bacteria : Microbial Degradation of Lignin-related Compounds (II)

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The most-probable-number technique indicated that soils and rumen contents of cattle were good habitats for organisms utilizing methoxylated aromatic acids or methanol. The number of vanillic acid-utilizers and methanol-utilizers were calculated to be at most 1.3×10^6 and 7.9×10^4 per gram of sample, respectively. Bacteria which grew on vanillic, veratric or p-anisic acid as a sole source of carbon were isolated from soil samples. These bacteria could not utilized methanol, formaldehyde, or formic acid as a sole source of carbon. Cell-free extracts of these organisms contained formaldehyde dehydrogenase, the activity of which increased when thece lls were grown on methoxylated compounds. Primary alcohol, methanol and formate dehydrogenases were not found in the extracts.The bacteria also possessed hydroxypyruvate reductase. Growth substrate had no clear effect on the enzyme formation.
公益社団法人日本生物工学会の論文
1981-02-25