SSU1-R, a Sulfite Resistance Gene of Wine Yeast, is an Allele of SSU1 with a Different Upstream Sequence
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概要
- 論文の詳細を見る
A sulfite resistance gene, SSU1-R, was cloned from a highly sulfite-resistant strain of Saccharomyces cerevisiae, Y-9, which was isolated as a contaminant from a wine must. The coding region sequence of SSU1-R was almost identical to that of SSU1, the gene that has been shown to be responsible for sulfite resistance. Although SSU1 is located on chromosome XV1 of S.cerevisiae, the upstream region of SSU1-R was homologous with a sequence of chromosome VIII. In our Northern analysis, an intense band was detected in Y-9 and K1-V1116 strains, both of which exhibit strong sulfite resistance, with and without sulfite in the medium. On the other hand, under the same experimental conditions, no band was detected in OC-2, a strain that exhibits weak sulfite resistance. Physical mapping of SSU1/SSU1-R showed that this sequence was on chromosome VIII of Y-9 and the two wine strains, K1-V1116 and WE14. OC-2 and twenty-three other wine, sake beer, shochu (Japanese distilled liquor), alcohol, bakery, and laboratory strains, had this sequence on chromosome XVI, and four other wine strains had it on both chromosomes. Thus, the difference in the upstream sequence of SSU1/SSU1-R seems to cause differences in the transcription rates and degree of sulfite resistance.
- 社団法人日本生物工学会の論文
- 1998-11-25
著者
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後藤(山本) 奈美
葛巻高原食品加工
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Goto‐yamamoto N
National Res. Inst. Brewing Higashi‐hiroshima Jpn
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Goto-yamamoto Nami
National Res. Inst. Of Brewing
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Hara Shodo
Kiku-masamune Sake Brewing Co.ltd.
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KITANO KAZUYOSHI
Saint Neige Wine Co.Ltd.,
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SHIKI KUNIO
Higashinada Ward Office
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YOSHIDA YUICHI
Kanto-Shinetsu Regional Taxation Bureau
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SUZUKI TAKASHI
Kanto-Shinetsu Regional Taxation Bureau
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IWATA TOMOKO
Osaka Regional Taxation Bureau
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YAMANE YOSHIHARU
Okinawa Regional Taxation Bureau
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Kitano Kazuyoshi
Saint Neige Wine Co.ltd.
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Goto‐yamamoto N
National Research Institute Of Brewing
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Goto‐yamamoto Nami
National Research Institute Of Brewing
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