Phenetic Clustering of Grapes (Vitis spp.) by AFLP Analysis

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概要

• 論文の詳細を見る

• For AFLP analysis of grapes, three different concentrations of restriction enzymes were examined to reduce artifact bands due to incomplete digestion or star activity of the restriction enzymes. Differences among band intensities were neglected, and all the detected bands were used in the analysis. A phenogram of 12 cultivars of Vitis vinifera, two cultivars of V.labrusca and two wild species, V.ficifolia var.lobata (V.thunbergii, Ebizuru) and V.coignetiae (Yamabudou) was obtained by UPGMA (unweighted pair group method arithmetic averages) based on the percentage of unshared AFLP bands of each sample pair, using four primer pairs. This phenogram revealed a clear separation of the tested species and proles with high bootstrap probabilities, except for the clusters with a longer phenetic distance in the Oriental cultivars of V.vinifera. The cluster of Occidental cultivars split into a cluster consisting of Cabernet Sauvignon and Cabernet franc, and another cluster consisting of Pinot noir, Pinot blanc, Chardonnay and Riesling. Among the Oriental cultivars, two Japanese cultivars, Koshu and Koshu-Sanjaku, formed a cluster. Thus, this phenogram agreed well with the classification based on morphological properties and geographical location.

• 日本育種学会の論文

著者

• 後藤(山本) 奈美

  葛巻高原食品加工

• Goto‐yamamoto N

  National Res. Inst. Brewing Higashi‐hiroshima Jpn

• Goto-yamamoto Nami

  National Res. Inst. Of Brewing

• Goto‐yamamoto N

  National Research Institute Of Brewing

• Goto‐yamamoto Nami

  National Research Institute Of Brewing

関連論文

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• SSU1-R, a Sulfite Resistance Gene of Wine Yeast, is an Allele of SSU1 with a Different Upstream Sequence
• Influence of Maceration Temperature in Red Wine Vinification on Extraction of Phenolics from Berry Skins and Seeds of Grape (Vitis vinifera)
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