Isolation and Identification of N, N-Dimethylformamide-Biodegrading Bacteria

概要

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N, N-Dimethylformamide (DMF)-using bacteria were isolated from soils in a factory which has produced DMF for anout 20 years. Isolates DM-15 and DM-82 resembled Paracoccus denitrificans, and isolate DM-81 does Pseudomonas aminovorans on the basis of morphological and chemotaxonomical cheracteristics. Methylobacterium sp. TH-15 and Mycobacterium sp. TH-35,reported previously as tetramethylammonium hydroxide (TMAH)-using bacteria, can use DMF. These five strains use DMF up to 2 or 3 wt%, and especially isolates DM-15 and DM-82 use DMF strongly. Dimethylamine, monomethylamine, and formic acid as intermediates from DMF are detected in the broth of flask cultures. Isolate DM-15 biodegrades 1% DMF in continuous culture at a dilution rate below 0.20 h^<-1> completely. This bacterium semms to be useful for the biodegradation of waste fluids containing DMF.
社団法人日本生物工学会の論文
1990-07-25