Isolation and Cultivation of Methanol-Utilizing Bacteria

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A large number of methanol-utilizing bacteria were isolated from a wide variety of natural sources in Japan. Isolates BNK-84,B-185 and BNS-25 which showed interesting characters of high growth rate, high optimum growth temperature, or low optimum growth pH were used for this study. Continuous cultures of these strains were carried out, and the feasibility of single cell protein production from methanol was studied.Optimum cultural conditions of strain BNK-84 were 38℃, pH 6.2-6.8 and a dilution rate of 0.2-0.6h^<-1>, those of strain B-185 were 40-42℃, pH 6.5-7.0 and a dilution rate of 0.1-0.33h^<-1>, and those of strain BNS-25 were below 32℃, pH 4.0-4.5 and a dilution rate of 0.10-0.17h^<-1>. Of these strains, strain BNK-84 seems to be the most suitable for commercial production of bacterial SCP from methanol in the cell yield, the growth rate, the contents of crude protein and amino acids, and the composition of amino acids. Strain B-185 showed an interesting character of high growth temperature, and strain BNS-25 exhibited an optimum growth pH of as low as 4.5. Strain BNK-84 showed the maximum specific growth rate, 0.60h^<-1> ; a cell yield, 45 wt% ; a content of crude protein, 86 wt% ; a content of amino acids, 70 wt% ; and the maximum content of nucleic acids, 20wt%. The methanol consumption rate per unit cell mass for maintenance, m, was about 0.04g methanol/g cell・h.Protein and anthrone-positive substances (APS) were detected in the broth supernatant of the three strains. Especially strain B-185 produced a large amount of protein, and its productivity was decreased by an increase of the dilution rate and the cultural temperature.
公益社団法人日本生物工学会の論文
1982-08-25

Isolation and Cultivation of Methanol-Utilizing Bacteria

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