Synthesis of Photolabile Caged Amino Acids for Measuring Amino Acid Transporters
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概要
- 論文の詳細を見る
Photolabile precursors (caged compounds) of amino acids such as Ala, Leu, Lys, and Ser were prepared by some simple reactions. These compounds were designed for the rapid, photochemically initiated release of amino acids. These amino acid transporters were expressed in Xenopus oocyte by injecting mRNA prepared from rat kidney. The electrical response of each transporter was examined by applying the amino acids and caged compounds before and after photolysis. Photolysis of the caged amino acids increased the electrical response of the facilitated amino acid transporters expressed in the oocyte. Consequently, these synthesized caged amino acids would be applicable to kinetic investigations on the transporters when combined with a pulsed laser or xenon arc flash lamp.
- 社団法人日本農芸化学会の論文
- 1992-07-23
著者
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Kamimura Akio
Department Of Applied Molecular Bioscience Graduate School Of Medicine Yamaguchi University
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Tanaka Daisuke
Department Of Clinical Oncology Aichi Cancer Center Hospital
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Kamimura A
Yamaguchi Univ. Yamaguchi
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Aoshima Hitoshi
Department Of Biology Physics And Informatics Faculty Of Science And Department Of Biological Chemis
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Tanaka Daisuke
Department Of Applied Chemistry
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Tanaka Daisuke
Department Of Chemistry Faculty Of Liberal Arts Yamaguchi University
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Tanaka Daisuke
Department of Anatomy, Keio University School of Medicine
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