The Sugar Specificity of a Na^+/Glucose Cotransporter from Rat Jejunum

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概要

• 論文の詳細を見る

• A cDNA for a Na^+/glucose cotransporter was cloned from a rat jejunum cDNA library. This transporter was expressed in Xenopus oocytes by injection of cRNA synthesized from the cDNA, and the transporter ability was electrophysiologically examined. The cotransporter had a very narrow sugar specificity. Only D-glucose, D-galactose, and some of their derivatives elicited significant electrical responses. These results of sugar specificity were compared with those of the H-+/hexose cotransporter of Chlorella. Dose-response relationships of several sugars followed a simple Michaelis-Menten type of kinetics. Both V_m and K_m were dependent on the sugars. Not only the affinity of sugars to the cotransporter but also the rate of conformational change of the cotransporter loaded with the sugar and Na^+, which translocates them from outside to inside, possibly depends on the sugar structure. The rate-limiting step of the transportation may be the conformational change, i.e., isomerization, of the cotransporter that translocates both the sugar and Na^+ from outside to inside.

• 社団法人日本農芸化学会の論文
• 1997-06-23

著者

• Yamada Mamoru

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• Yamada Mamoru

  Department Of Biochemistry Faculty Of Agriculture Yamaguchi University

• Izu Hanae

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Yokoyama T

  Graduate School Of Agricultural Science Tohoku University

• Aoshima Hitoshi

  Department Of Biology Physics And Informatics Faculty Of Science And Department Of Biological Chemis

• YOKOYAMA Terufumi

  Department of Biology, Physics and informatics, Faculty of Science, Yamaguchi University

• TANIZAKI Junko

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• Tanizaki J

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Yamaguchi Toshiyasu

  Marine Biochemistry Laboratory Gradate School Of Agricultural Science Tohoku University

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