Analysis of Products of the Escherichia coli Genomic Genes and Regulation of Their Expressions : An Applicable Procedure for Genomic Analysis of Other Microorganisms

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概要

• 論文の詳細を見る

• A partial library of the Escherichia coli genomic genes has been constructed, in which each clone has part of a genomic gene fused in frame with the lacZ gene in addition to its promoter and operator. DNA of randomly selected clones was sequenced, and the resultant deduced N-terminal amino acid sequences showed that 17 out of 26 genes analyzed encode unknown proteins. Genomic locations of the cloned genes and their expressional regulations under the aerobic and anaerobic conditions were also analyzed. These results suggest that this library is useful for the global analysis of the E. coli genomic genes and that this strategy may be applicable to the genomic analysis of other microorganisms.

• 社団法人日本農芸化学会の論文
• 1994-01-23

著者

• Yanai S

  Staff Inst. Ibaraki Jpn

• Talukder Ali-Azam

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• Yanai Satoshi

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• Yamada Mamoru

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• Yamada Mamoru

  Department Of Biochemistry Faculty Of Agriculture Yamaguchi University

• Yamada M

  Japan Marine Sci. And Technol. Center Yokosuka Jpn

• Talukder Ali-azam

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

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• Analysis of Products of the Escherichia coli Genomic Genes and Regulation of Their Expressions : An Applicable Procedure for Genomic Analysis of Other Microorganisms
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