Inhibition of DNA Topoisomerase I by Dihydrotanshinone I, Components of a Medicinal Herb Salvia miltiorrhiza Bunge

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概要

• 論文の詳細を見る

• Dihydrotanshinone I induced topoisomerase I-mediated DNA cleavage in vitro as strongly as camptothecin, but topoisomerase II-mediated DNA cleavage was not affected. In a DNA relaxation assay using calf thymus DNA topoisomerase I and supercoiled pBR322 plasmid DNA, dihydrotanshinone I reduced topoisomerase I-mediated DNA relaxation in a dose-dependent manner. Heat treatment (65℃) of the reaction mixture containing dihydrotanshinone I and topoisomerase I resulted in a substantial reduction in DNA cleavage, suggesting topoisomerase I and dihydrotanshinone I may form reversible cleavable complex to induce DNA damage. A DNA unwinding assay using T_4DNA ligase showed that dihydrotanshinone I is a very weak DNA intercalator. These results suggest that dihydrotanshinone I inhibits the catalytic activity of topoisomerase I by the formation of a cleavable complex and at least in part through the intercalation into DNA.

• 社団法人日本農芸化学会の論文
• 1999-08-23

著者

• Lee Sang-han

  Department Of Food Science & Technology Kyungpook National University

• Lee Sang-han

  Department Of Microbiology College Of Natural Science Kyungpook National University

• LEE Dong-Sun

  Department of Microbiology, College of Natural Science, Kyungpook National University

• KWON Gi-Seok

  Department of Life Resource Science, College of Natural Science, Andong National University

• LEE Hong-Kum

  Marine Microbiology Research Laboratory, Biological Oceanography Division, Korea Ocean Research & De

• WOO Joo-Hyung

  Department of Microbiology, College of Natural Science, Kyungpook National University

• KIM Jong-Guk

  Department of Microbiology, College of Natural Science, Kyungpook National University

• KONG Soon-Duck

  Department of Microbiology, College of Natural Science, Kyungpook National University

• Kim Jong-guk

  Department Of Microbiology College Of Natural Science Kyungpook National University

• Lee Dong-sun

  Department Of Microbiology College Of Natural Science Kyungpook National University:(present Address

• Lee Dong-sun

  Department Of Chemistry Seoul Women's University

• Lee Hong-kum

  Marine Microbiology Research Laboratory Biological Oceanography Division Korea Ocean Research &

• Kwon Gi-seok

  Department Of Life Resource Science College Of Natural Science Andong National University

• Woo Joo-hyung

  Department Of Microbiology College Of Natural Science Kyungpook National University

• Kong Soon-duck

  Department Of Microbiology College Of Natural Science Kyungpook National University

• Lee Sang-Han

  Department of Food Science & Biotechnology, Kyungpook National University

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