Separation, Characterization, and Specificity of α-Mannosidases from Vigna umbellata

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概要

• 論文の詳細を見る

• Information about the specificity of glycosidase enzymes is important since it affects their use for characterization and synthesis of oligosaccharides. Two α-mannosidases (EC 3.2.1.24), I and II, were isolated from rice beans (Vigna umbellata). The native molecular weight of both isozymes was estimated to be 329,000,but pIs of form I were 5.03-5.34 and pIs of form II were 5.46-6.20. The two isozymes were characterized in terms of optimal pH and temperature, effects of metal ions, inhibition by swainsonine and 1-deoxymannojirimycin, and kinetic parameters for pnitrophenyl-α-D-mannopyranoside and Man α(1-2)Man. Both enzymes were more specific towards Man α(1-2) Man in both hydrolysis and synthesis, but their hydrolytic specificities towards Man α(1-3) [Man α(1-6)] Man were different.

• 社団法人日本農芸化学会の論文
• 1998-04-23

著者

• Jisnuson Svasti

  Department Of Biochemistry Faculty Of Science Mahidol University:laboratory Of Biochemistry Chulabho

• Svasti Jisnuson

  Department Of Biochemistry And Center For Excellence In Protein Structure & Function Faculty Of

• Patjaraporn WONGVITHOONYAPORN

  Department of Biochemistry, Faculty of Science, Mahidol University

• Christopher BUCKE

  Division of Biotechnology, School of Biological and Health Sciences, University of Westminster

• Christopher Bucke

  Division Of Biotechnology School Of Biological And Health Sciences University Of Westminster

• Patjaraporn Wongvithoonyaporn

  Department Of Biochemistry Faculty Of Science Mahidol University

• Wongvithoonyaporn Patjaraporn

  Department of Biochemistry, Faculty of Science, Mahidol University

• Bucke Christopher

  Division of Biotechnology, School of Biological and Health Sciences, University of Westminster

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