Biological Activity of Brain-derived Neurotrophic Factor with Mismatched Disulfide Linkages Produced by Escherichia coli
スポンサーリンク
概要
- 論文の詳細を見る
E. coli cells harboring an expression plasmid with a brain-derived neurotrophic factor (BDNF) gene obtained from rat BDNF cDNA were sonicated and centrifuged to obtain a precipitate containing BDNF. Ten different proteins of BDNF were purified from the precipitate and the three disulfide linkages of six proteins were identified. Those disulfide structures were different from that of authentic BDNF and the biological activities of BDNF with mismatched disulfide linkages (EC_<50> of 2 to 15ng/ml) were much lower than that of authentic BDNF (EC_<50> of 30 pg/ml). The BDNF with mismatched disulfide linkages inhibited the biological activity of authentic BDNF.
- 社団法人日本農芸化学会の論文
- 1996-06-23
著者
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Shimizu N
Advanced Research Laboratory Hitachi Ltd.
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Ishibashi Tadashi
Advanced Research Laboratory Hitachi Ltd.
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SHIMIZU Norio
Advanced Research Laboratory, Hitachi, Ltd.
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FUKUZONO Shinichi
Advanced Research Laboratory, Hitachi, Ltd.
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FUJIMORI Kiyoshi
Advanced Research Laboratory, Hitachi, Ltd.
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PARK Kyungmoon
Advanced Research Laboratory, Hitachi, Ltd.
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TAKESHITA Tomoko
Advanced Research Laboratory, Hitachi, Ltd.
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Park K
Advanced Research Laboratory Hitachi Ltd.
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Park Kyungmoon
Advanced Research Lab. Hitachi Ltd.
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Shimizu N
Materials And Structures Laboratory Tokyo Institute Of Technology
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Takeshita T
Advanced Research Laboratory Hitachi Ltd.
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Takeshita Tomoko
Advanced Research Laboratory Hitachi Ltd.
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Fukuzono Shin'ichi
Advanced Research Laboratory Hitachi Ltd.
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Fujimori K
Advanced Research Laboratory Hitachi Ltd.
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Fujimori Kiyoshi
Advanced Research Laboratory Hitachi Ltd.
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Shimizu Norio
Advanced Research Lab. Hitachi Ltd.
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ISHIBASHI Tadashi
Advanced Research Laboratory, Hitachi, Ltd.
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