Expression of Brain-Derived Neurotrophic Factor by Bacillus subtilis

概要

論文の詳細を見る
An expression and secretion plasmid for brain-derived neurotrophic factor (BDNF) was constructed with a constitutive promoter and the Bacillus subtilis subtilisin sequence. For the first time, BDNF expression and secretion by recomdinant B. subtilis were showed by measuring the biological activity of the medium with dorsal root ganglion neurons of 8-d chick embryos. The presence of BDNF in the medium was revalidated by the observation of lower biological activity with the BDNF antibody, but the estimated BDNF level was very low. In addition, 1.5 times higher biological activity was achieved in the glucose medium than in the glucose-free medium because of catabolite repression of protease expression resulting in lower proteolytic degradation of the product.
社団法人日本生物工学会の論文
1996-12-25