Cultivation of Escherichia coli Harbouring Hybrid Plasmids

概要

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Cultivation of Escherichia coli transformed with a hybrid plasmid pTREZl, containing a β-galactosidase (β-gal) gene under the control of the trp promoter, was investigated to develop a control method for effective production of heterologous proteins in bacteria. When glucose in the culture broth was consumed, dissolved oxygen (DO) concentration and respiratory quotient (RQ) rapidly increased. Then the inducer, 3-β-indolylacrylic acid, and casamino acids were added to induce the trp operon. β-Gal production per cell mass reached about 5 U/mg. In addition, RQ rapidly increased when β-gal production ceased, and proved to be a better indicator of E. coli cultivation than DO concentration.
社団法人日本生物工学会の論文
1987-02-25