Application of L-Glutamate to L-Proline Fermentation by Corynebacterium acetoacidophilum

概要

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L-Proline-producing mutants were screened from Corynebacterium acetoacidophilum ATCC 13870. Proline accumulation in test tube cultivation by TA-20 strain was most effectively stimulated by addition of L-glutamate, rising from 11.8 g/l to 30.8 g/l in the presence of 2% of L-glutamate. Use of radioactively labeled glutamate demonstrated that the increased amount of proline derived from the quantitative conversion of glutamate to proline. Unlike other strains reportes previously, this strain showed to dependence on biotin concentration or high (more than 4%) salt concentration. In 2-l jar fermentors, proline productivity was also stimulated by the addition of glutamate. The amount of proline accumulated increased with the concentration of glutamate in the range of 2% to 6%. With 6% of glutamate and 2.5% of ammonium sulfate, 108.3 g/l of L-proline was accumulated in 42 h of cultivation.
公益社団法人日本生物工学会の論文
1987-04-25