Purification and Characterization of β-Galactosidase from Scopulariopsis sp.

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概要

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• A strain of Scopulariopsis sp. which produced high activity of extracellular β-galactosidase was isolated from soil, and the crude enzyme was propared by wheat bran culture of the microorganism. The crude enzyme was further purified by precipitation with ammonium sulfate, and DEAE-cellulose, and CM-cellullose column chromatographies. The purified β-galactosidase was electrophoretically homogeneous and its molecular weight was estimated to be 95,000 by both Sephadex G-200 gel filtration and SDS-polyacrylamide gel electrophoresis. The optimum pH of the enzyme activity was between 3.6 and 5.0 and maximum activity appeared at 65℃.

• 公益社団法人日本生物工学会の論文
• 1980-02-25

著者

• Pastore Glaucia

  Universidade Estadual De Campinas Faculdade De Engenharia De Alimentos (unicamp)

• Pastore Glaucia

  Universidade Estadual De Compinas Faculdade De Engenharia De Alimentos(unicamp)

• PARK YONG

  Universidade Estadual de Campinas, Faculdade de Engenharie de Alimentos(UNICAMP)

• Park Yong

  Universidade Estadual De Campinas Faculdade De Engenharie De Alimentos(unicamp)

• Park Yong

  Universidade Estadual De Campinas Faculdade De Engenharia De Alimentos

関連論文

• Purification and Characterization of β-Galactosidase from Scopulariopsis sp.
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• Immobilization of Scopulariopsis β-Galactosidase to DEAE-Cellulose with Glutaraldehyde and Some Properties of the PReparation Obtained
• Microflora in Beiju and Their Biochemical Characteristics
• Purification and Characterization of Acid α-Amylase from Paecilomyces sp.
• Induction of High Amyloglucosidase-producing Mutant from Aspergillus awamori
• Conversion of Cane Bagasse to Compost and Its Chemical Characteristics

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