A Novel Conjugative Plasmid Conferring Multiple-antibiotic Resistance Detected in Epiphytic Strains of Enterobacter cloacae
スポンサーリンク
概要
- 論文の詳細を見る
Several epiphytic strains of Enterobacter cloacae isolated from mulberry leaves were resistant to antibiotics such as streptomycin, kanamycin, ampicillin, tetracycline and chloramphenicol, and harbored a 100-kb plasmid designated pMUL1. Plasmid profile analysis of spontaneous mutants derived from Ent. cloacae MUL1 and MUL1 (RSF1010) suggested that pMUL1 confers resistance to multiple antibiotics. Southern blot analysis using probes of five antibiotic-resistance genes against EcoRI-digested DNA from pMUL1 and defective pMUL1s (mutants) revealed that all these genes were located within a 24-kb region of pMUL1 and that some genes were assigned to the defective plasmids. A similar antibiotic-resistance plasmid was detected in several orchid-pathogenic strains of Ent. cloacae, but not in the type-culture strain (JCM 1232) or strains of Ent. cloacae of insect-origin. Strains MUL1 and MUL1 (RSF1010) were then mated with epiphytic Erwinia spp. and Pseudomonas spp. on filters, respectively. Several recipient strains of epiphytic Er. herbicola simultaneously acquired plasmid pMUL1 and the phenotype of multiple-antibiotic resistance. Thus, pMUL1 was verified to be conjugative and to encode genes for multiple-antibiotic resistance, including genes homologous to the strA-strB of the nonconjugative IncQ plasmid RSF1010. These findings suggest that epiphytic Ent. cloacae may play a role in the dissemination of antibiotic-resistance genes among epiphytic bacteria and plant pathogenic bacteria.
- 日本植物病理学会の論文
- 2002-08-25
著者
-
Watanabe Kenji
National Inst. Materials Sci. (nims) Ibaraki Jpn
-
Sato Mamoru
National Institute Of Agrobiological Resources
-
Sato Mamoru
National Institute Of Agrobiological Sciences (owashi Campus)
-
Watanabe Kenji
National Institute Of Agrobiological Sciences (owashi Campus)
関連論文
- Photon Correlation in GaAs Self-Assembled Quantum Dots
- Hetero-Epitaxial Growth of ZnO Film by Temperature-Modulated Metalorganic Chemical Vapor Deposition
- Possible vector insect of mulberry dwarf phytoplasma, Tautoneura mori Matsumura
- Distribution patterns of mulberry dwarf phytoplasma in reproductive organs, winter buds, and roots of mulberry trees
- Multidrug-resistance plasmid of Enterobacter cloacae : transfer to Erwinia herbicola on the phylloplane of mulberry and weeds
- Epiphytic Bacterium, Erwinia ananas, Commonly Isolated from Rice Plants and Brown Planthoppers (Nilaparvata lugens) in Hopperburn Patches
- Luminescence Characteristics and Annealing Effect of Tb-Doped AlBNO Films for Inorganic Electroluminescence Devices
- First-Principles Study of Various Hexagonal BN Phases(Condensed matter : electronic structure and electrical, magnetic, and optical properties)
- Synthesis of Cubic and Hexagonal Boron Nitrides by Using Ni Solvent under High Pressure
- Rapid Degradation of Carbaryl by Two Novel Strains of Arthrobacter spp. Isolated from Forest Soil
- Efficient Production of Ethylene by Pseudomonas syringae pv. Glycinea When Causes Halo Blight in Soybeans(Microbiology & Fermentation Industry)
- The Damping Behavior of Ni Added Mn-Cu Damping Alloys
- Biocontrol of Phytophthora Root Rot of Angelica Trees by Enterobacter cloacae and Serratia ficaria Strains
- Enhanced Infection of a Nucleopolyhedrovirus in a Lepidopteran Pest (Spilosoma imparilis) by Spindles of a Coleopteran Entomopoxvirus (EPV) (Anomala cuprea EPV)
- Pseudomonas syringae pv. solidagae pv. nov., the Causal Agent of Bacterial Leaf Spot of Tall Goldenrod Solidago altissima L.
- A Novel Conjugative Plasmid Conferring Multiple-antibiotic Resistance Detected in Epiphytic Strains of Enterobacter cloacae
- Chemical Vapor Deposition of C Isotopically Enriched Polycrystalline Diamond (SELECTED TOPICS IN APPLIED PHYSICS : Diamond Semiconductors : from Materials to Devices)
- Electrical Spin Injection into Graphene through Monolayer Hexagonal Boron Nitride
- Fabrication and Characterization of High-Mobility Graphene p--n--p Junctions Encapsulated by Hexagonal Boron Nitride
- Fabrication and Characterization of High-Mobility Graphene p-n-p Junctions Encapsulated by Hexagonal Boron Nitride (SELECTED TOPICS IN APPLIED PHYSICS : Nano Electronics and Devices : Characterization and Control of Nano Surfaces and Interfaces)