6. シロイヌナズナの新規インドール-3-酢酸代謝物の探索および同定

概要

論文の詳細を見る
Although it has been considered to date that a large amount of endogenous indole-3-acetic acid (IAA) in plant is present as conjugated forms, the identified compounds account for only a minor part of the estimated total IAA. In this study, we searched for novel IAA-conjugates and its oxidative catabolites, in order to better understand the IAA metabolism in Arabidopsis. The extract of Arabidopsis (Col-0) seedlings was partially purified using solid-phase extraction cartridge. The presence of IAA and 2-oxoindole-3-acetic acid (OxIAA) metabolites was monitored by LC-ESI-MS/MS systems operated in multiple reaction monitoring (MRM) mode. By comparing the LC behavior and MS/MS data with synthetic standards, two new oxidative conjugates, OxIAA-Phe and OxIAA-Glc were identified. Furthermore, the presence of the conjugates of hydroxy IAA with Asp, Glu, Phe and Tyr was suggested. In order to determine their substitution position of hydroxy group on the indole ring, a series of amino acid conjugates of 4-OH-IAA, 5-OH-IAA, 6-OH-IAA and 7-OH-IAA were respectively synthesized. These synthetic standards were compared with natural products in terms of LC behavior and MS/MS data for identification.
植物化学調節学会の論文
2005-10-13