F-0723 高張液による浸透圧変化を受けた細胞の生存率(J22-3 生体関連の熱・流体工学(3))(J22 生体関連の熱・流体工学)

概要

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It has been believed that exposure to hypertonic solution during freezing is the major cause of the post-thaw cellular injury after freezing with low cooling rate. To develop a fundamental understanding of cell damage due to hypertonic solution, we have developed a new perfusion technique that isolates the effect of exposure to hypertonic solutions from other effects associated with ice crystals. Using human prostatic adenocarcinoma cells we characterized post-hypertonic survival as a function of hypertonic solution concentration, the time of exposure, and the rate of increase or decrease in solution concentration. The post-hypertonic viability depended on the increase rate of concentration as well as the solution concentration and the time of exposure.
一般社団法人日本機械学会の論文
2001-08-22