微細一酸化窒素電極による臓器内一酸化窒素の計測

概要

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A newly developed nitric oxide (NO) microsensor was applied to NO measurements in isolated organs. The NO sensor was inserted into the vascular media of an isolated canine femoral artery, perfused with a Krebs-Henseleit solution. NO concentration in the media increased linearly with increasing perfusion flow rate (wall shear stress). NO production was attenuated by N^G-mono-methyl-L-arginine (L-NMMA). A dramatical change in NO concentration in the perfusate from an isolated beating rat heart was observed after 1-min flow suspension. NO concentration increased markedly during the first 30 sec of reperfusion and returned to the level before flow suspension after 3-4 min. NO release was enhanced by increasing perfusion pressure (70 to 100 cmH_2O), i.e., perfusion flow rate (wall shear stress). The newly developed NO microsensor showed satisfactory performance for measurement of NO in the vascular media and in the perfusate from the isolated heart.
日本医療機器学会の論文
1996-01-01