真菌の免疫学的活性物質の研究 : 2.Candida菌体成分の免疫学的活性.(特に皮内反応活性)について

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In the previous paper, it was reported that the active principles for delayed skin reaction of coccidioidin and histoplasmin were considered to be a protein in nature. In this paper, it was attempted to define the chemical property of immunologically active substances of Candida. Organisms of C. albicans, Ca 13 strain, were repeatedly extracted with 0.1 N KOH solution and then with 1 N KOH solution. Precipitates were obtained from these extracts by HC1 precipitation at pH 4.0. Supernates were deproteinized with TCA and precipitated with ethanol. These fractions were skin test active and their activities were markedly reduced by the treatment with proteolytic enzymes. The crude protein fraction was further purified by zone electrophoresis and two purified polysaccharide fractions (D_3-1 and PS-1) were obtained by DEAE-cellulose column chromatography or by gel filtration with Sephadex G 200. The purified protein fraction contained 14.12% N and still 2.7% sugar. The purified polysaccharide fractions, D_3-1 and PS-1, contained 0.66% N and 97.2% suger, and 0.77% N and 95.3% sugar, respectively. PS-1 was immunologically homogeneous in agar gel precipitation and showed a symmetric peak in ultracentrifugal analysis (6.1 S). The skin test activity of the purified protein fraction was more than 100 times that of the purified polysaccharide fraction. When the purified protein or the crude polysaccharide fraction was injected with complete Freund's adjuvant into foot pads of guinea pigs, these fractions were capable of sensitizing animals for delayed skin reaction. However, the purified polysaccharide and the papain treated crude antigens failed to sensitize animals. These results indicated that the active principle of skin test antigen in Candida was protein, too.
日本アレルギー学会の論文
1968-01-30

真菌の免疫学的活性物質の研究 : 2.Candida菌体成分の免疫学的活性.(特に皮内反応活性)について

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