Candida菌体成分による即時および遅延型反応 : I.ヒトにおける皮膚・気管支反応

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The aim of this work was to study the antigenic components present in Candida albicans and to clarify the relationships between the dermal and bronchial reactions of immediate and delayed type provoked with the fractions from the organisms in man. Organisms of Candida albicans were repeatedly extracted with 0.1 N KOH and then with 1 N KOH. These extracts were precipitated with HCl at pH 4.0 and the supernates were deproteinized with TCA and precipitated with ethanol. These crude polysaccharide fractions were used as crude extracts. The crude extracts were further purified by the treatment of proteolytic enzyme followed by filtration with Sephadex G-200 to obtain purified polysaccharide fraction (PS). Precipitates from original extracts with HCl at pH 4.0 were purified by zone electrophoresis to obtain purified protein fraction (Prot.). PS contained 0.77% N and 95.3% sugar and Prot. Contained 14.2% N and 2.7% sugar. Skin tests with these fractions revealed: PS elicited immediate reaction, Prot. Showed immediate, delayed, or both reactions, and crude extracts provoked immediate, intermediate, delayed, or some combinations of these reactions. The incidences of positive immediate skin reactions were 37.4% in asthma, 27.3% in allergic rhinitis, 7.1% in tubeculosis and 9.0% in other nonallergic diseases. There was a significantly high incidence of immediate neactions in the atopic group for PS or crude extract. However, the atopic individuals exhibited no greater incidence of delayed neactions than the nonatopic individnals for Prot or crude cxtrats' Sixteen inhalation tests were performed in 13 asthmatic patients. The bronchial and systemic reactions were studied by spirometry, bodyplethysmography, measurements of body temperature, respiration rates, white blood counts, etc. Two among 5 subjects which were challenged with PS showed immediate reaction only, 1 among 4 who inhaled Prot. Showed delayed reaction only. Some challenged with one of both fractions, crude extracts or commercially available antigen extracts, reacted both immediately and delayedly. One of 2 subjects with negative skin reactions showed also negative bronchial reaction when crude extracts were inhaled. It may be concluded from the above observations that the antigenic substances of immediate skin hypersensitivity are included in PS of the C. albicaus while Prot. Contains those of delayed. This seems to be true on the antigenicity of the components of Candida albicans for bronchial reaction. Immediate allergic reaction is kown to induce bronchoconstriction in asthmatic patients. It is likely that delayed type reaction with exudation, edema and cell infiltration in respiratory tract may also cause dyspnea in patients with hyperreactivity in the bronchus and bronchioles.
日本アレルギー学会の論文
1970-02-28

Candida菌体成分による即時および遅延型反応 : I.ヒトにおける皮膚・気管支反応

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