こんにゃく喘息の起因抗原に関する研究

概要

論文の詳細を見る
In the past ten years, several investigators have attemped to isolate and purify the active allergens in "Maiko", a dust from Amorphophalus Konjac (which is also offending allergens of typical allergic asthma in human beings in Japan) by various chemical techniques and electrophoresis in starch. For the purpose of the present study, three water soluble fractions has been separated by fractional precipitation from "Maiko" extract. Precipitation was carried out at 0-40% saturation, 60-90% saturation and 0-90% saturation with ammonium sulfate. The antigenic specificity of these farctions has been shown by direct skin tests and passive transfer studies, bis-diazotized-benizdine method, gel diffusion method with the sera of Maiko sensitive patients, and by Schultz-Dale tests with iluem from sensitized guinea pigs. The results obtained were as follows: The 60-90% fraction was skin reactive, but the 0-40% fraction was noreactive. However, in bronchial provocation test, the 0-40% fraction was more reactive than the 0-60% fraction. Hemagglutination and geldiffusion data showed that both hemagglutinating and precipitating anti-gens are present in each fraction. Studies by the Schultz-Dale test schowed that the 0-40% fraction and the 0-60% fraction has different antigenic specificity. The 90% fraction was reactive in all tests.
日本アレルギー学会の論文
1966-10-25