Expression of IL-1β mRNA in Mice after Whole Body X-Irradiation
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概要
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IL-1β is a stimulator of hematopoietic and inflammatory systems, and also acts as a radioprotector. After whole-body exposure to sublethal doses of ionizing radiation, the IL-1β mRNA level in spleen cells increases for a short time prior to regeneration of the spleen. We analyzed spleen cells of C3H/He mice after whole-body irradiation with 3 Gy x-rays to determine the cause of this short-term increase in the transcription level. An increase in the level of the message in spleen cells, found by Northern blot hybridization, reached its peak 5 to 7 days after irradiation. There was a low correlation between the curves of the mRNA level and the ratio of monocyte/macrophage lineage cells; a typical source of the message. Spleen macrophages that produce a large amount of the message were found 7 days after irradiation in an in situ hybridization experiment in which heterogeneous spleen cell populations were used. In contrast, spleen cells had no detectable levels of macrophages rich in IL-1β mRNA before and 17 days after irradiation. Additionally, the population of message-rich cells was 9.4% of the total number of monocytes/macrophages in the spleen. These results suggest that the short-term increase in IL-1β mRNA is a result of the heterogeneous differentiation of a subpopulation of spleen macrophages before regeneration of the spleen.
- 日本放射線影響学会の論文
著者
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SUZUKI Gen
Div. Clin. Res. Radiat. Health. Natl Inst. Radiat. Sci.
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Tanaka Izumi
Treatment Research Team Medical Treatment For High Dose Exposure Research Group Research Center Radi
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Tanaka Izumi
放射線医学総合研究所緊急被ばく医療研究センター
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Nemoto Kumie
放射線医学総合研究所生体制御研究グループ
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Ohtsu Hiroshi
放射線医学総合研究所生体制御研究グループ
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Yoshida Kazuko
放射線医学総合研究所
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TSUNEOKA KAZUKO
放射線医学総合研究所生体制御研究グループ
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Tanaka Izumi
放射線医学総合研究所生体制御研究グループ
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Suzuki Gen
Department Of Clinical Studies Radiation Effect Research Foundation
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Suzuki Gen
放射線影響研究所 臨床研究部
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SUZUKI GEN
放射線医学総合研究所生体制御研究グループ
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Suzuki Gen
Health Care Center International University Of Health And Welfare Clinic
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Yoshida Kazuko
放射線医学総合研究所生体制御研究グループ
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Suzuki Gen
Division Of Radiation Health National Lnstitute Of Raciological Sciences
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Suzuki Gen
Division Of Clinical Research And Radiation Health National Institute Of Radiological Sciences
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