Immediate-early, Transient Induction of the Interleukin-1β Gene in Mouse Spleen Macrophages by Ionizing Radiation
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概要
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In murine spleen cells, x ray irradiation induces the expression of the IL-1β gene at multiple phases of the peak time. We analyzed the immediate-early phase of IL-1β mRNA accumulation. To determine the lineage of cells that showed the immediate response to irradiation, normal spleen cells were analyzed by Northern blotting and in situ hybridization after separation by magnetic antibodies against specific cell-surface antigens. Although most of the spleen macrophages continuously expressed a low level of IL-1β mRNA, a portion of the macrophage population transiently accumulated large amounts of IL-1β message immediately after irradiation. A macrophage-like leukemia cell line that resembles these inducible macro-phages was identified. A similar immediate-early and transient increase in the IL-1β mRNA level occurred when cultured spleen cells were irradiated with a low dose (3 Gy) of x rays. In contrast, the x ray-inducible expression of the IL-1β gene was immediate and continuous, not transient, in spleen cells from whole-body irradiated mice. Results of the run-on transcription assay and the determination of the decrease in the message using cultured spleen and macrophage-like leukemia cells indicated that x ray irradiation appears to activate the transcription of the IL-1β gene and partially stabilize the message. The results show that the x ray-induced immediate-early accumulation of IL-1β mRNA is regulated at both the transcriptional and post-transcriptional levels in an as yet unidentified population of spleen macrophages.
- 日本放射線影響学会の論文
著者
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石原 弘
放射線医学総合研究所生体制御研究グループ
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Tanaka Izumi
放射線医学総合研究所緊急被ばく医療研究センター
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Nemoto K
Division Of Pathology And Physiology National Institute Of Radiological Sciences
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Ohtsu Hiroshi
Division Of Pathology And Physiology National Institute Of Radiological Sciences
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Yoshida K
Division Of Pathology And Physiology National Institute Of Radiological Sciences
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Tanaka I
放射線医学総合研究所生体制御研究グループ
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NEMOTO KUMIE
Division of Pathology and Physiology, National Institute of Radiological Sciences
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TSUNEOKA KAZUKO
放射線医学総合研究所生体制御研究グループ
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CHEERAMAKARA CHEERARATANA
放射線医学総合研究所生体制御研究グループ
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YOSHIDA KAZUKO
Division of Pathology and Physiology, National Institute of Radiological Sciences
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Nemoto Kazuhiro
Maizuru Marine Observatory Japan Meteorological Agency
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Tanaka Izumi
放射線医学総合研究所生体制御研究グループ
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Yoshida Kazuko
Division Of Biology And Oncology National Institute Of Radiological Sciences
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Tsuneoka K
Bioregulation Research Group National Institute Of Radiological Sciences
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Cheeramakara Cheeraratana
Visiting Scientist From The Institute Of Tropical Medicine Mahidol University
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