Recovery of Haemopoietic Stem Cells of W/W^v Mice after Irradiation: In Vivo and in Vitro Studies

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The growth kinetics of haemopoietic cells after irradiation was compared between the normal (+/+) and the genetically anemic W/W^v mice both in vivo and in vitro. The parameters studied were the total cellularity and the number of granulocyte/macrophage precursor cells (CFUc). The onset of haemopoiesis in vivo after irradiation with 150 R of X-rays was significantly delayed in W/Wv mice compared to that in +/+ littermates. In the culture of W/W^v marrow, haemopoietic cells could recover after 50 R-irradiation but not after irradiation with more than 100 R, whereas the +/+ marrow culture restored normal haemopoiesis soon after irradiation. The number and concentration of CFUc within the W/W^v marrow culture showed a temporary ncrease after irradiation, followed by a gradual decrease which preceded the depletion of differentiated cells in suspension. These results suggest that the differentiation potency of W/W^v stem cell is normal but the capacity for self-renewal is defective.