AcetyIcholinesterase活性検出増強法によるHirschsprung病の組織化学的診断

概要

論文の詳細を見る
Histochemical staining of acetylcholinesterase (AChE) activity of rectal suction biopsy is a reliable method in the diagnosis of Hirschsprung's disease. Diagnosis of Hirchsprung's disease can be established by the increased AChE positive nerve fibers in the inucosa and submucosa of the rectum. But there have been several reports on the presence of false negative reaction. Some of these may depend upon technical fault such as an insufficient incubation solution. We have applied an improved AChE staining technique using rubeanic acid to 32 children with constipation. Eleven cases were confirmed to have Hirschsprung's disease by this method. No false positive or negative reaction was encountered. The sites of AChE activities were observed as black deposits with sharp contrast. This new method will be expected to be widely used for the basic and clinical study of Hirschsprung's disease.
日本小児外科学会の論文
1984-06-20