INDUCTION OF UNSCHEDULED DNA SYNTHESIS IN HAIRLESS MOUSE EPIDERMIS BY ULTRAVIOLET LIGHT

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概要

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• The activity of ultraviolet(UV)light to induce unscheduled DNA synthesis(UDS)was investigated in hairless mouse epidermis by means of an in vivo-in vitro assay using a liquid scintillation counting method. Groups of three to five 8-week-old female hairless mice were irradiated with UV-B or UV-A, then skin samples were taken and cultured individually in medium containing[3^H]thymidine with or without hydroxyurea(HU)for 2 hr. DNA of the epidermis was extracted, and incorporation of[3^H]thymidine and the DNA content were determined with a liquid scintillation counter and a fluorescence spectrophotometer, respectively. Induction of UDS was judged in terms of the UDS index[(the ratio of DNA synthesis in the presence of HU to that in its absence)×100]. UV-B increased the UDS index 1 hr after irradiation of 500 J / m^2, which corresponds to approximately 1 minimal erythema dose or 1 minimal edema dose, and showed a dose-dependent increase up to 17-fold in the UDS index at irradiation doses of 500 to 2, 000 J / m^2. In a time-coures study, UV-B also increased replicative DNA synthesis(RDS)48 hr after irradiation at 1, 000 J / m^2. On the other hand, UV-A did not increase the UDS index at irradiation doses of 2×10^5 to 8×10^5 J / m^2. These results show that induction of UDS by UV irradiation depends on wavelength, and an increase of RDS in the epidermis exposed to UV-B irradiation appears after induction of UDS.

• 日本トキシコロジー学会の論文
• 2000-02-28

著者

• FURIHATA Chie

  College of Science and Engineering, Aoyama Gakuin University

• Mori M

  Ajinomoto Co. Inc. Kawasaki Jpn

• Mori Mayumi

  Life Science Research Center Shiseido Co. Ltd.

• MORI Masaaki

  Life Science Research Center, Shiseido Co., Ltd.

• KOBAYASHI Hiroshi

  Life Science Research Center, Shiseido Co., Ltd.

• KATSUMURA Yoshio

  Life Science Research Center, Shiseido Co., Ltd.

• SUGIYAMA Chiyomi

  Life Science Research Center, Shiseido Co., Ltd.

• NAGANUMA Masako

  Scientific Research Deparment, Shiseido Co., Ltd., 3-9-1 Nishi-gotanda

• Furihata Chie

  College Of Science And Engineering Aoyama Gakuin University

• Naganuma Masako

  Scientific Research Deparment Shiseido Co. Ltd. 3-9-1 Nishi-gotanda

• Mori M

  Institute For Cosmic Ray Research University Ot Tokyo

• Sugiyama Chiyomi

  Life Science Research Center Shiseido Co. Ltd. 1050 Nippa-cho

• Katsumura Y

  Life Science Research Center Shiseido Co. Ltd.

• Furihata Chie

  College of Science and Engineering, Aoyama-Gakuin University

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