血清中テオフィリン濃度測定における Particle-Enhanced Turbidimetric Inhibition Immunoassay の評価

概要

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We evaluated the particle-enhanced turbidimetric inhibition immunoassay (PETINIA) for the measurement of theophylline in serum. The coefficients of variation in both within-day(n=10) and between-day (n=10) precision studies were less than 4%, and the analytical recoveries were 98-103% at the concentrations of 5, 10 and 20μg/ml of theophylline. Good correlations were observed among assay results by PETINIA, high-performance liquid chromatography (HPLC) and fluorescence polarization immunoassay (FPIA) for 120 serum specimens collected from patients with chronic obstructive pulmonary disease receiving theophylline therapy. The regression lines obtained were : PETINIA=1.034xHPLC-0.404 (r=0.994, Sy/x=o.57μg/ml), PETINIA=.,037xFPIA-0.539 (r=0.995, Sy/x=0.51μg/ml). The cross reactivity in PETINIA was also studied with 11 compounds structurally related to theophylline. None of these compounds exhibited significant cross reactivity (less than 5%) except for 1,3-dimethyluric acid (11%). From these results, we could conclude that PETINIA is a useful method for therapeutic monitoring of serum theophylline having good precision and accuracy, and high specificity.
日本医療薬学会の論文
1987-12-20