高速液体クロマトグラフィーによる体液中パラコートの分析

概要

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Paraquat was directly assayed in biological fluids by high-performance liquid chromatography (HPLC) with a UV detector using a reversed phase column packed with CHEMCOSORB3-0DS-H (4.6 I.D. × 75mm) and ion-pair chromatography. Mobile phase consisted of 0.02M sodium dodecylsulfate and 0.1M triethylamine in 31% acetonitrile-water, with the pH adjusted to 4.0 with phosphoric acid. Ethylparaquat (1,1'-diethyl-4,4'-bipyridylium diiodide) was used as the internal standard. Samples (500μl each) of the biological fluids were each added to the internal standard solution (50μl) and mixed with 50% trichloric acid solution (50μl) for deproteinization. After the centrifugation of the mixture, 20μl of its supernatant was analyzed by HPLC. The recovery of paraquat from the biological fluids spiked with this agent in the concentration of 0.1-10μg/ml was 93.0-107.0% in serum, 93.2-103.8% in urine, 100.4-109.1% in intestinal juice 93.4-108.3% in gastric juice. The linearlity of calibration curve was evident for paraquat in the concentration of 0.1-10μg/ml in these biological fluids. This method was not in disagreement with chromatograms of therapeutic drugs and other compounds. The detection limit of paraquat in the analysis of biological fluids was 0.01μ9/ml (S/N=2).
日本医療薬学会の論文
1987-08-20