高速液体クロマトグラフィーを用いた血漿中L-DOPAの定量

概要

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The L-DOPA levels in plasma were determined by high performance liquid chromatography. Separation of L-DOPA from plasma was achieved by alumina adsorption using tris-HCl buffer (pH 8.6) without deproteinization. Adsorption of L-DOPA onto alumina showed liquid-solid equilibriums with constant rates at both adsorption and extraction steps. For this reason, the recovery of L-DOPA from plasma varied with alumina amounts, acidity and volume of the eluate used. Maximal recovery (89%) was achieved when 50 mg of alumina and 1 ml of eluate of I M AcOH were used. Norepinephrine and epinephrine, as well as L-DOPA, were assayed by this method, and linear caribration curves were obtained as little as 2000 pg/ml for norepinephrine and epinephrine, and 5μg/ml for L-DOPA. Reproducibility was demonstrated by a day-to-day coefficient of variation based on the change of assay value for standard plasma sample containing 1μg/ml of L-DOPA. The day-to-day coefficient of variation was less than 4%, and the within-run coefficient of variation was less than 3%. The interference by other probable catechols in plasma such as dopamine and carbidopa which is often administered with L-DOPA were not found. The method involving alumina extraction established in this study has general applicability in very accurately determining L-DOPA, norepinephrine and epinephrine in plasma.
日本医療薬学会の論文
1984-10-20