ヘテロジニアスEIAキット(MARKIT^<[○!R]>)による血中カルバマゼピンとバルプロ酸の濃度測定

概要

論文の詳細を見る
Heterogeneous enzyme immunoassay kit "MARKIT" (Dainippon Pharmaceutical Co., Ltd.) for the determination of carbamazepine (CBZ) and valproic acid (VPA) in the blood of patients with epilepsy was evaluated. The plasma CBZ and VPA levels measured by the MARKIT method and gas liquid chromatography method (GLC) were correlated well; the correlation coefficients of the MARKIT method were 0.95 for CBZ (n=30) and 0.98 for VPA (n=30) and coefficients of variation intra- and inter-assays for 3 levels of CBZ or VPA were less than 10%. Calibration of plasma CBZ or VPA levels by using programmed micro-computer was more rapid and accurate than that by the manual method, where individual variation for generation of a standard curve was omitted. Relationship between plasma CBZ or VPA levels and daily dosage of VPA or CBZ was unpredictable due to individual difference amoung patients and, therefore, therapeutic monitoring of these drugs was necessary.
日本医療薬学会の論文
1983-12-20