Dual Inoculation of Peanut with Glomus sp.and Bradyrhizobium sp.Enhanced the Symbiotic Nitrogen Fixation as Assessed by ^<15>N-Technique
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概要
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The response of peanut (Arachis hypogaea L.) to inoculation with vesicular-arbuscular mycorrhizal (VAM) fungi (Glomus etunicatum) and Bradyrhizobium sp.was studied in pots by the acetylene reduction activity (ARA) and 'A-value' methods. The soil used was a Light-coloured Andosol and the treatments consisted of the inoculation of VAM fungi only, inoculation of Bradyrhizobium only, dual inoculation of VAM fungi and Bradyrhizobium and control, under non-sterilized and sterilized soil conditions. In the non-sterilized soil the ARA and nitrogen fixation determined by the 'A-value' method increased significantly only by dual inoculation of VAM fungi and Bradyrhizobium at 100 days after planting (DAP), but no significant difference was observed at 70 DAP. In the case of dual inoculation, 75% of the nitrogen of the plant was derived from fixation whereas the plants inoculated only with Bradyrhizobium derived 68% of their nitrogen from fixation and the control plants, 64%. Amount of P in plant increased significantly only by dual inoculation with VAM fungi and Bradyrhizobium. In the sterilized soil a highly significant increase in the ARA was observed of the dual inoculation at all the sampling times. Nitrogen fixation determined by the A-value technique and N and P contents in plant also increased significantly by dual inoculation. Results obtained by the A-value method showed that plants with dual inoculation derived 68% of their nitrogen from fixation while the plants inoculated only with Bradyrhizobium, 38%. From our this study we conclude that nitrogen fixation as well as N and P contents in peanut increased significantly only by dual inoculation with VAM fungi and Bradyrhizobium.
- 社団法人日本土壌肥料学会の論文
著者
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Sakamoto Kazunori
Faculty Of Horticulture Chiba University
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Yoshida Tetsushi
Faculty of Agriculture, Kochi University
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Sakamoto K
Chiba Univ.
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Khan Monowar
Faculty of Horticulture, Chiba University
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Yoshida Tomio
Faculty of Horticulture, Chiba University
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Khan M
Bangladesh Institute Of Nuclear Agriculture
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Khan Monowar
Faculty Of Horticulture Chiba University
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Yoshida Tomio
Faculty Of Horticulture Chiba University
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Yoshida T
Faculty Of Agriculture Kochi University
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Sakamoto K
Faculty Of Horticulture Chiba University
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