EVALUATION OF N_2 FIXATION BY APPLYING ^<15>N LABELED PLANT MATERIAL AND AMMONIUM SULFATE

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Effect of different <15>^N labeled sources on the estimation of N_2 fixation was investigated. The combination of <15>^N labeled ammonium sulfate, <15>^N labeled plant material, and <15>^N labeled ammonium sulfate with unlabeled plant material, was examined in pot experiments. Two cultivars of soybean (Glycine max) and one of mungbean (Vigna radiata) were used. No significant difference was observed among the treatments for the estimation of N_2 fixation. This was due to the homogeneity and stability of the <15>^N abundance soil which resulted in a similar N uptake from the soil by the N_2 fixing and reference crops. The plant yield, total N uptake and amount of N_2 fixed were higher in the Yellow Soil than in the Andosol. The amount of N_2 fixed was strongly influenced by the plant growth and consequently it affected the plant yield. The slow decomposition of plant material in the Andosol resulted in a low yield in both the N_2 fixing and reference crops. Thus, the artificial decrease of the available N content in soil, by application of plant material, did not stimulate N_2 fixation but suppressed plant growth and N_2 fixation.
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