Characterization of Phosphatase in Marigold Roots Infected with Vesicular-arbuscular Mycorrhizal Fungi

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概要

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• The phosphatase specific to the infection of vesicular-arbuscular mycorrhizal fungi (Glomus etunicatum Becker & Gerdemann) in marigold (Tagetes patula L.) roots was investigated in relation to the mycorrhizal growth promotion in host plant. Mycorrhizal and non-mycorrhizal marigold plants were cultured in a growth chamber, and the enzyme solution was extracted from 2- to 10-week-old plant roots. The infection-specific phosphatase (ISPase) was detected in the mycorrhizal root extract from the beginning of the infection (4-week-old plant) by an electrophoretical technique. The activity increased as the infection rate increased, and decreased at the stationary phase of the host growth when the infection rate was still high. The effect of phosphorus (P) fertilization on the ISPase activity and the mycorrhizal growth promotion in host was investigated in 4- to 8-week-old plants using two kinds of liquid fertilizers with different P concentrations. The plants without P-fertilization showed more increase in the shoot fresh weight (SFW) of mycorrhizal plant and higher ISPase activity (from 0 to given week) were highly correlated with the increase in the SFW of the mycorrhizal plant. The ISPase was partially purified by ammonium sulfate, hydrophobic chromatography, and gel filtration, and characterized as follows : the optimum pH was 7.5, 1mM phosphate ion inhibited half of the activity, the enzyme hydrolyzed inosine 5'-diphosphate effectively and polyphosphate moderately. The possibility of using ISPase as an indicator for the growth response of mycorrhizal plants and the metabolic role of this enzyme were discussed.

• 一般社団法人日本土壌肥料学会の論文

著者

• Yoshida Tomio

  Faculty Of Horticulture Chiba University

• Ezawa Tatsuhiro

  Faculty Of Horticulture Chiba University

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